There are many variables to consider when producing recombinant proteins and more specifically recombinant antigens. These variables include construct design, affinity tags, expression system, purification scheme, formulation, and characterization.
Here we will focus on the use of affinity tags in recombinant antigen production. To know more about recombinant antigens, you can also check out Boster Bio featured products online.
When developing recombinant antigen expression constructs and purification workflows, it is worth considering affinity labeling. The addition of affinity markers to recombinant antigens can simplify downstream purification and testing. However, affinity markers can make immunization for antibody production difficult.
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Affinity markers can, for example, alter the structure of the antigen, rendering certain epitopes inaccessible for B cell recognition. This reduces the immunogenicity of the antigen, resulting in decreased antibody production. In addition, the tag itself can trigger an immune response.
This means that the resulting preparation is a mixture of antibodies against the intended target marker and antigen. There are many companies available that provide the best antibody products online.
Larger affinity markers may cause more problems than smaller markers, but this cannot be guaranteed. To avoid complications in antibody production, constructs can be designed so that the affinity label can be removed during the purification process by protease degradation.